Development and Validation of Rp-hplc Method for the Simultaneous Estimation of Diacerein and Aceclofenac in Dosage Form

A simple Reverse phase liquid chromatographic method has been developed and subsequently validated for simultaneous determination of Diacerein and Aceclofenac in combination. The separation was carried out using a mobile phase consisting of dipotassium hydrogen phosphate buffer of pH 4.5, Acetonitrile and methanol in the ratio of 40: 40: 20. The column used was Phenomenex Luna C18, 5μ, 250 mm × 4.6 mm id with flow rate of 1.0 ml / min using UV detection at 265 nm. The described method was linear over a concentration range of 80-120 μg/ml and 160-240 μg/ml for the assay of Diacerein and Aceclofenac respectively. The retention times of Diacerein and Aceclofenac were found to be 1.780, and 3.035 min respectively. Results of analysis were validated statistically. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Diacerein and Aceclofenac bulk drug and in its pharmaceutical dosage form.